Heffelfinger SC, et al. SK HEP-1 : a human cell line of endothelial origin. In Vitro Cell. Dev. Biol. 28A: 136-148, 1992. PubMed: 1371504. Gucev ZS, et al. Evidence for insulin-like growth factor (IGF)-independent transcriptional regulation of IGF binding protein-3 by growth hormone in SKHEP-1 human hepatocarcinoma cells.
80% RPMI 1640 + 20% FBS split confluent culture 1:10 to 1:20 about 1-2 times a week using trypsin/EDTA; cells grow fast; seed out at ca. 3-4 x 106 cells/80 cm 2 at 37C with 5% CO2 about 15-18 x 106 cells/175 cm 2; doubling time of ca. 30 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule; contamination was eliminated with Mycoplasma Removal Agent, then.
For Sk?Hep?1 cells it is possible to observe a decrease in glucose concentration for up to 120 hours of cultivation. Lactate, which is a product of glucose metabolism, showed an increase in the concentration at the start of cultivation, reaching 35 mM at 96 hours and.
80% RPMI 1640 + 20% FBS split confluent culture 1:10 to 1:20 about 1-2 times a week using trypsin/EDTA; cells grow fast; seed out at ca. 3-4 x 106 cells/80 cm 2 at 37C with 5% CO2 about 15-18 x 106 cells/175 cm 2; doubling time of ca. 30 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule; contamination was …
I would greatly appreciate if you could help me in a problem that I am facing the last days about the SK-Hep1 cell line. The original vial (purchased by ATCC) was grown with a sufficient rate and …
SK-HEP-1 Xenograft Model; SNU-398 Xenograft Model; SMMC-7721 Xenograft Model; … Doubling Time : Bacteria Growth Rate Formula: N t = N 0 * ( 1 + r) t. N t: The amount at time t N 0: The amount at time 0 r: Growth rate t: Time passed. Please send the details of your project to info@altogenlabs.com, or call 512-433-6177 and we will be happy to …
